Bio-Safety Level 3 Core

The Ragon BSL3 core provides biosafety level-3 laboratory space, training, and consultation for academic and industrial researchers working with risk group-3 pathogens, such as M. tuberculosis, SARS-CoV-2, Chikungunya virus, West Nile virus, Powassan virus, Japanese Encephalitis virus, Dabie bandavirus, Heartland virus and Bhanja virus. 

Researchers are required to complete 30 hours of BSL3 specific training to become certified BSL3 core users. Training covers special facility containment, BSL-3 specific PPE, and safety regulations specific to risk group-3 pathogens.


Amy Barczak

Scientific Director


Julie Boucau


Yong (Mike) Xie


Services and Pricing

The Ragon BSL3 is one of the largest (2200 square feet) and most heavily used BSL3 facility in the region, with an excellent safety track record. The lab is equipped with dedicated 12-color high-speed cell sorter and microscopy equipment, as well as cell culture hoods and standard cell and molecular biology resources.

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Selected Publications

SARS-CoV-2 Virologic Rebound With Nirmatrelvir-Ritonavir Therapy : An Observational Study

Edelstein GE, Boucau J, Uddin R, Marino C, Liew MY, Barry M, Choudhary MC, Gilbert RF, Reynolds Z, Li Y, Tien D, Sagar S, Vyas TD, Kawano Y, Sparks JA, Hammond SP, Wallace Z, Vyas JM, Barczak AK, Lemieux JE, Li JZ, Siedner MJ

Ann Intern Med . 2023 Nov 14:M23-1756 PubMed Read on Journal Site

Immunopeptidomics reveals determinants of Mycobacterium tuberculosis antigen presentation on MHC class I.

Leddy O, White FM, Bryson BD.

Elife. 2023 Apr 19;12:e84070 PubMed Read on Journal Site

TLR2 is non-redundant in the population and subpopulation responses to Mycobacterium tuberculosis in macrophages and in vivo.

Jani C, Solomon SL, Peters JM, Pringle SC, Hinman AE, Boucau J, Bryson BD, Barczak AK.

mSystems. 2023 Aug 31;8(4) PubMed Read on Journal Site

SARS-CoV-2 infection of human pluripotent stem cell-derived liver organoids reveals potential mechanisms of liver pathology.

Richards A, Friesen M, Khalil A, Barrasa MI, Gehrke L, Jaenisch R.

iScience. 2022 Oct 21;25(10) PubMed Read on Journal Site

FcγR-mediated SARS-CoV-2 infection of monocytes activates inflammation

Caroline Junqueira, Ângela Crespo, Shahin Ranjbar, Luna B de Lacerda, Mercedes Lewandrowski, Jacob Ingber, Blair Parry, Sagi Ravid, Sarah Clark, Marie Rose Schrimpf, Felicia Ho, Caroline Beakes, Justin Margolin, Nicole Russell, Kyle Kays, Julie Boucau, Upasana Das Adhikari, Setu M Vora, Valerie Leger, Lee Gehrke, Lauren A Henderson, Erin Janssen, Douglas Kwon, Chris Sander, Jonathan Abraham, Marcia B Goldberg, Hao Wu, Gautam Mehta, Steven Bell, Anne E Goldfeld, Michael R Filbin, Judy Lieberman

Nature, 606(7914), 04/04/22 PubMed Read on Journal Site

Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteins

Fengwen Zhang, Trinity M Zang, Eva M Stevenson, Xiao Lei, Dennis C Copertino, Talia M Mota, Julie Boucau, Wilfredo F Garcia-Beltran, R Brad Jones, Paul D Bieniasz

Proceedings of National Academy of Science, 119 (41) 10/11/2022 PubMed Read on Journal Site

Duration of Shedding of Culturable Virus in SARS-CoV-2 Omicron (BA.1) Infection

Julie Boucau, Caitlin Marino, James Regan, Rockib Uddin, Manish C Choudhary, James P Flynn, Geoffrey Chen, Ashley M Stuckwisch, Josh Mathews, May Y Liew, Arshdeep Singh, Taryn Lipiner, Autumn Kittilson, Meghan Melberg, Yijia Li, Rebecca F Gilbert, Zahra Reynolds, Surabhi L Iyer, Grace C Chamberlin, Tammy D Vyas, Marcia B Goldberg, Jatin M Vyas, Jonathan Z Li, Jacob E Lemieux, Mark J Siedner, Amy K Barczak

New England Journal of Medicine 2022, June 29 PubMed Read on Journal Site

Virologic Features of Severe Acute Respiratory Syndrome Coronavirus 2 Infection in Children

Yonker LM, Boucau J, Regan J, Choudhary MC, Burns MD, Young N, Farkas EJ, Davis JP, Moschovis PP, Bernard Kinane T, Fasano A, Neilan AM, Li JZ, Barczak AK

J Infect Dis . 2021 Dec 1;224(11) PubMed