Charles Evavold

Charles Evavold, PhD

Principal Investigator

Core Member and Early Independence Fellow

Ragon Institute of MGH, MIT and Harvard


400 TS 758

Synthetic immunity and cell death regulation

Research Overview

The Evavold lab studies fundamental cellular decision making towards different cell fates using cell death programs as a model system. We have a particular interest in the intersection of microbiology and metabolism with impact on host cell survival or death.

Regulated cell death can serve different functions within the innate immune system. For example, immunologically silent cell death can remove unnecessary, damaged, or pre-malignant cells during development and homeostasis. Alternatively, during the early containment of a pathogen, inflammatory cell death can alert the immune system to a foreign invader. However, inflammatory cell death can become pathogenic during systemic infection or contribute to sterile autoimmunity and chronic inflammation. Conversely, acquired resistance to cell death programs can promote cancer progression or resistance to chemotherapies. Dysfunction of cell death regulation may underlie much of the pathology related to human diseases. Thus, we aim to uncover novel regulation in these processes using precision screening that combines genomic or chemical perturbations with synthetic biology models of discrete signaling nodes.


Dr. Charlie Evavold received his PhD in Immunology from Harvard University. He received his BS in Physics and Astronomy with a double major in Chemistry from Emory University. Charlie is one of the inaugural Ragon Early Independence Fellows on the basic science track.

Selected Publications

Control of gasdermin D oligomerization and pyroptosis by the Ragulator-Rag-mTORC1 pathway

Charles L Evavold, Iva Hafner-Bratkovič, Pascal Devant, Jasmin M D’Andrea, Elsy M Ngwa, Elvira Boršic, John G Doench, Martin W LaFleur, Arlene H Sharpe, Jay R Thiagarajah, Jonathan C Kagan

Cell 2021, Volume 184, Issue 17, Pages 4495-4511.e19 Download PDF PubMed Read on Journal Site

HDAC6 mediates an aggresome-like mechanism for NLRP3 and pyrin inflammasome activation

Venkat Giri Magupalli, Roberto Negro, Yuzi Tian, Arthur V Hauenstein, Giuseppe Di Caprio, Wesley Skillern, Qiufang Deng, Pontus Orning, Hasan B Alam, Zoltan Maliga, Humayun Sharif, Jun Jacob Hu, Charles L Evavold, Jonathan C Kagan, Florian I Schmidt, Katherine A Fitzgerald, Tom Kirchhausen, Yongqing Li, Hao Wu

Science 2020, Vol 369, Issue 6510 Download PDF PubMed Read on Journal Site

Inflammasomes: threat-assessment organelles of the innate immune system

Charles L Evavold, Jonathan C Kagan

Immunity 2019, Volume 51, Issue 4, p609–624 Download PDF PubMed Read on Journal Site

Defying death: the (w)hole truth about the fate of GSDMD pores

Charles L Evavold, Jonathan C Kagan

Immunity 2019, Volume 50, Issue 1, p15-17 Download PDF PubMed Read on Journal Site

How inflammasomes inform adaptive immunity

Charles L Evavold, Jonathan C Kagan

Journal of molecular biology 2018, Volume 430, Issue 2, p217-237 Download PDF PubMed Read on Journal Site

The pore-forming protein gasdermin D regulates interleukin-1 secretion from living macrophages

Charles L Evavold, Jianbin Ruan, Yunhao Tan, Shiyu Xia, Hao Wu, Jonathan C Kagan

Immunity 2018, Volume 48, Issue 1, p35-44. e6 Download PDF PubMed Read on Journal Site

PF4 promotes platelet production and lung cancer growth

Ferdinando Pucci, Steffen Rickelt, Andita P Newton, Christopher Garris, Ernesto Nunes, Charles Evavold, Christina Pfirschke, Camilla Engblom, Mari Mino-Kenudson, Richard O Hynes, Ralph Weissleder, Mikael J Pittet

Cell Reports 2016, Volume 17, Issue 7, p1764-1772 Download PDF PubMed Read on Journal Site

SCS macrophages suppress melanoma by restricting tumor-derived vesicle–B cell interactions

Ferdinando Pucci, Christopher Garris, Charles P Lai, Andita Newton, Christina Pfirschke, Camilla Engblom, David Alvarez, Melissa Sprachman, Charles Evavold, Angela Magnuson, Ulrich H Von Andrian, Katharina Glatz, Xandra O Breakefield, Thorsten R Mempel, Ralph Weissleder, Mikael J Pittet

Science 2016, Volume 352, Issue 6282, p242-246 Download PDF PubMed Read on Journal Site